Fig. 5: PLD2-induced p53 inactivation is mediated by SIRT1. | Experimental & Molecular Medicine

Fig. 5: PLD2-induced p53 inactivation is mediated by SIRT1.

From: Phospholipase D2 is a positive regulator of sirtuin 1 and modulates p53-mediated apoptosis via sirtuin 1

Fig. 5

a Effect of SIRT1 depletion on PLD2-induced inactivation of p53 transcriptional activity. A549 cells were transfected with the indicated constructs and p53-responsive Bax or Noxa-luciferase reporter. The luciferase activity was measured. Protein expression levels were analyzed by immunoblotting. b Effect of SIRT1 depletion on the acetylation of p53 suppressed by PLD2. A549 cells were transfected with PLD2 and shSIRT1 and treated with 30 μM etoposide and 500 nM TSA. Lysates were analyzed by immunoblotting using the indicated antibodies. The results are representative of at least three independent experiments and are shown as the mean ± SEM. **P < 0.01, ***P < 0.001, n.s., nonsignificant.

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