Fig. 2: MHY-1685 improved the differentiation capacity of senescent hCSC. | Experimental & Molecular Medicine

Fig. 2: MHY-1685 improved the differentiation capacity of senescent hCSC.

From: Human cardiac stem cells rejuvenated by modulating autophagy with MHY-1685 enhance the therapeutic potential for cardiac repair

Fig. 2: MHY-1685 improved the differentiation capacity of senescent hCSC.

a Endothelial tube formation ability of senescent hCSCs was determined by a tube formation assay (*p < 0.05 vs. vehicle). Scale bar: 50 µm. b Heatmap analysis of the positive regulation of gene expression related to angiogenesis. c Enrichment of angiogenesis-related GO terms of genes that were significantly regulated in the MHY-1685-primed hCSCs. d Expression of CDH5, Flk1, and PECAM1 after differentiation into endothelial cell lineage as examined by qRT-PCR (*p < 0.05 vs. undifferentiated hCSCs, #p < 0.05 vs. vehicle). e Vascular smooth muscle cell differentiation ability as evaluated by fluorescent immunocytochemistry using the myocyte marker α-SMA. α-SMA (green), DAPI (blue). (*p < 0.05 vs. vehicle). Scale bar: 50 μm. f Expression of ACTA2, CNN1, and GATA6 after differentiation into the vascular smooth muscle cell lineage as examined by qRT-PCR (*p < 0.05 vs. undifferentiated hCSCs, #p < 0.05 vs. vehicle). Data are shown as the mean ± S.E.M.

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