Fig. 4: BLT2 knockout suppresses KrasG12D-driven lung tumor formation and IL-6 production.
a Representative images of lung lesions and b H&E staining of lung tissue. Scale bars, 500 μm. c Airway cellular infiltration in the indicated experimental groups. The arrows indicate infiltrated cells. Scale bars, 100 μm. d Representative images of BALF cytospin preparations isolated from each group. The results are representative of five independent experiments with similar results. e The number of lung tumors in the mice was counted. The data are presented as the mean ± SD; n = 5. *p < 0.05 according to Student’s t-test. f BLT2 protein levels in lung lysates as measured by western blotting. The results are representative of three independent experiments with similar results. Band intensities were quantified using ImageJ and are expressed as the fold change relative to the control value. g The average number of BALF cells per field of view was determined. The results are presented as the mean ± SD values; n = 5. ***p < 0.001 with Student’s t-test. Lung tissue extracts were collected to analyze (h) LTB4 and (i, j) IL-6 by ELISAs. The data are presented as the mean ± SD; n = 5. *p < 0.05 and **p < 0.01 according to Student’s t-test. N.S. not significant.
