Fig. 3: The UBXN8 promoter region is highly methylated in RUNX1-RUNX1T1+ cell lines.
From: Epigenetic silencing of UBXN8 contributes to leukemogenesis in t(8;21) acute myeloid leukemia
a Methylation level at the UBXN8 gene promoter region (–1500 to –1 bp, TSS up 1.5 kb) as analyzed by MCC-Seq was obviously higher in SKNO-1 cells than in SKNO-siAE cells. b Schematic representation of the distribution of the CpG dinucleotides along the UBXN8 promoter region (–1500 to –1 bp, TSS up 1.5 kb). The horizontal line (F-R) indicates the location of the primers used in the BSP assays. c A genomic BSP assay was performed to profile the methylation pattern of the CpG dinucleotides located along the RUNX1-binding site. Black circles and empty circles denote methylated and unmethylated CpG dinucleotides, respectively. d The mRNA and protein expression of UBXN8 was determined in cells treated with 1.0 µM DAC. Error bars indicate the SD from three different assays.
