Fig. 2: Verification of the differences in cell subsets of PBMCs in AD patients.
From: Single-cell RNA sequencing reveals B cell–related molecular biomarkers for Alzheimer’s disease
a Heat map of differentially expressed genes in samples from AD patients and normal controls and (b) KEGG analysis of pathways involved in upregulated genes and downregulated genes. c t-SNE shows the top 6 cell subsets in all samples and (d) quantification of the proportion of cells in each sample. e, f The t-SNE map shows the different distribution of 6 clusters in normal, early AD, and late AD patients. The data were presented as the mean. The heatmap was generated by the pheatmap R package, and the rows were clustered. Pearson distance measurement assigns values to the clustering rows. The bubble diagram was generated by the ggplot R package. The color of the bubbles varies from red to green. The redder the bubbles are, the greater the -log10(p value), that is, the smaller the p value. The larger the bubble is, the more genes are enriched. N1, Normal-1; N2, Normal-2; EA1, Early AD-1; EA2, Early AD-2; LA1, Late AD-1; LA2, Late AD-2.
