Fig. 3: CU06-1004 prevents infiltration of inflammatory cells and apoptosis of CMs and CMECs. | Experimental & Molecular Medicine

Fig. 3: CU06-1004 prevents infiltration of inflammatory cells and apoptosis of CMs and CMECs.

From: CU06-1004 enhances vascular integrity and improves cardiac remodeling by suppressing edema and inflammation in myocardial ischemia–reperfusion injury

Fig. 3

a Representative images of neutrophils in the infarct zone at 48 h after reperfusion and a summary of their quantification. The number of neutrophils was quantified by counting multilobed nuclei. n = 6. *P < 0.05 vs. vehicle. †P < 0.05 vs. low CU06-1004. Scale bars: 100 µm. b Representative images of neutrophils stained with MPO in the infarct zone and a summary of their quantification. MPO (red) and DAPI (blue). n = 6. *P < 0.05 vs. vehicle. †P < 0.05 vs. low CU06-1004. Scale bars: 100 µm. c Representative images of M1 macrophages in the infarct zone and a summary of their quantification. CD68 (green), iNOS (red) and DAPI (blue). n = 6. *P < 0.05 vs. vehicle. †P < 0.05 vs. low CU06-1004. Scale bars: 100 µm. d Representative images of M2 macrophages in the infarct zone and a summary of their quantification. CD68 (green), CD206 (red), and DAPI (blue). n = 6. *P < 0.05 vs. vehicle. †P < 0.05 vs. low CU06-1004. Scale bars: 100 µm. e Representative images of apoptotic CMs in the infarct zone and a summary of their quantification. TUNEL (green), cTnT (red) and DAPI (blue). n = 6. *P < 0.05 vs. vehicle. †P < 0.05 vs. low CU06-1004. Scale bars: 200 µm. f Representative images of apoptotic CMECs in the infarct zone and a summary of their quantification. TUNEL (green), CD31 (red) and DAPI (blue). n = 6. *P < 0.05 vs. vehicle. †P < 0.05 vs. low CU06-1004. Scale bars: 200 µm. Data are shown as the mean ± SEM.

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