Fig. 1: TRIM45 expression was upregulated after cerebral I/R injury. | Experimental & Molecular Medicine

Fig. 1: TRIM45 expression was upregulated after cerebral I/R injury.

From: TRIM45 causes neuronal damage by aggravating microglia-mediated neuroinflammation upon cerebral ischemia and reperfusion injury

Fig. 1: TRIM45 expression was upregulated after cerebral I/R injury.The alternative text for this image may have been generated using AI.

a qRT-PCR assay indicating TRIM45 expression in cerebral tissues at the indicated time points post-middle cerebral artery occlusion/reperfusion; n = 4 per time point. b Mouse brain homogenates were extracted at the indicated time points following cerebral I/R injury. Western blot assay (above) and quantification (blow) showing TRIM45 expression; n = 4 per time point. c Homogenates of primary microglia subjected to oxygen and glucose deprivation (OGD) and reoxygenation at the indicated time points were extracted. Western blot assay (above) and quantification (blow) showing TRIM45 expression; n = 4 per time point. d Primary microglia were subjected to oxygen and glucose deprivation (OGD) and reoxygenation at the indicated time points. Immunofluorescence indicating TRIM45 expression; TRIM45 (red), and 4’,6-diamidino-2-phenylindole (DAPI; blue, nuclei). Scale bars, 20 μm. e Immunofluorescence indicating TRIM45 expression in the cerebral cortex, hippocampal CA1 region, and striatum of adult mice subjected to MCAO surgery for 1 h and reperfusion for 24 h; TRIM45 (red), Iba-1 (green) and nuclei (DAPI), scale bars, 40 μm. Data are presented as the mean ± S.E.M. and were analyzed by one-way ANOVA followed by Dunnett’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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