Fig. 2: TRIM45 enhances the NF-κB signaling pathway through TAB2 and TAK1. | Experimental & Molecular Medicine

Fig. 2: TRIM45 enhances the NF-κB signaling pathway through TAB2 and TAK1.

From: TRIM45 causes neuronal damage by aggravating microglia-mediated neuroinflammation upon cerebral ischemia and reperfusion injury

Fig. 2: TRIM45 enhances the NF-κB signaling pathway through TAB2 and TAK1.

a qRT-PCR showing the mRNA levels of proinflammatory genes and chemokines, such as IL-1β, IL-6, TNF-α, Cxcl1, and Ccl2, in primary cultured microglia transfected with lentivirus encoding shTRIM45 or the scramble control and then subjected to OGD/R after 48 h. b The secretion levels of IL-1β, IL-6, TNF-α, Cxcl1, and Ccl2 were determined by ELISAs of primary cultured microglia. c, d Western blot assay (c) and quantification (d) indicating NF-κB p65 levels in the cytoplasm and nucleus of primary cultured microglia. e, f Primary cultured microglial cells were transfected with lentivirus encoding shTRIM45 or the scramble control and then subjected to OGD/R after 48 h. Immunofluorescence assay (e) and quantification (f) indicated NF-κB p65 levels in the cytoplasm and nucleus. NF-κB p65 (green), nuclei (DAPI), Scale bars, 40 μm. Data in the (d, f) were analyzed by two-way ANOVA followed by Tukey’s post hoc test, and all others were analyzed by one-way ANOVA followed by Dunnett’s post hoc test. Data are presented as the mean ± S.E.M. from at least three independent experiments. n.s. for P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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