Fig. 6: Model of dynamic alternative splicing regulation of the TUBB3 gene.

Five kinds of transcripts (T0–T4) were identified between exon 5 and exon 7. Three (T0, T1, and T2) were generated by exon-skipping events, and two (T3 and T4) were produced by intron-retention events. Red boxes represent unique sequences compared with that of the T0 transcript. The positions of premature stop codons are shown as red stop signs. The green Pac-Man denotes isoforms subject to nonsense-mediated decay (NMD). Only the T0 transcript is predicted to avoid NMD, whereas the others are likely to be subjected to transcriptional degradation due to premature stop codons.