Fig. 6: miR-98-3p participates in PDE-induced inhibition of osteogenic differentiation in the F1-F3 generation by targeting the decrease in JAG1/Notch1 signaling. | Experimental & Molecular Medicine

Fig. 6: miR-98-3p participates in PDE-induced inhibition of osteogenic differentiation in the F1-F3 generation by targeting the decrease in JAG1/Notch1 signaling.

From: The miR-98-3p/JAG1/Notch1 axis mediates the multigenerational inheritance of osteopenia caused by maternal dexamethasone exposure in female rat offspring

Fig. 6

a Schematic representation of a predicted binding site of miR-98-3p in the 3’-UTR of JAG1 mRNA and the mutant JAG1 3’-UTR. b The luciferase activity was determined using the Dual Luciferase Reporter System (n = 3). c Immunohistochemistry and MOD analysis of JAG1 and Notch1 in the primary ossification center of fetal rats (n = 5), scale bar = 100 μm. d Immunohistochemistry and MOD analysis of femur JAG1 and Notch1 in 8-week-old rats of the F1-F3 generations (n = 5), scale bar = 10 μm. e Western blot analysis of JAG1/Notch1 expression in the BMSCs treated with 500 nM dexamethasone and miR-98-3p inhibitor (n = 3). f RT–qPCR analysis of the expression of osteogenic marker genes in the BMSCs treated with 500 nM dexamethasone and the JAG1 plasmid (n = 3). g Mineral deposition was indicated by Alizarin red staining (n = 3). h ALP staining was performed on Day 7 of osteogenic differentiation (n = 3). JAG1 Jagged1; Con control; MOD mean optical density; miR-NC miRNA negative control; PDE prenatal dexamethasone exposure; Dex dexamethasone; Runx2 Runt-related transcription factor 2; OCN osteocalcin; COL1A1 α1 chain of type I collagen gene. Mean ± S.E.M., *P < 0.05, **P < 0.01 vs. the control.

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