Fig. 6: Has2 deficiency in hepatic stellate cells alleviates CCl4-induced ALI. | Experimental & Molecular Medicine

Fig. 6: Has2 deficiency in hepatic stellate cells alleviates CCl4-induced ALI.

From: Hyaluronan synthase 2, a target of miR-200c, promotes carbon tetrachloride-induced acute and chronic liver inflammation via regulation of CCL3 and CCL4

Fig. 6: Has2 deficiency in hepatic stellate cells alleviates CCl4-induced ALI.The alt text for this image may have been generated using AI.

C57BL/6 mice were injected intraperitoneally with CCl4 or corn oil (Con). Liver samples were harvested 24 h after CCl4 injection. a qRT–PCR analysis of Has2 mRNA expression (Con, n = 5; CCl4, n = 7). **P < 0.01 indicates a significant difference versus the Con group. b qRT–PCR analysis of miR-200c expression in the livers of Con- and CCl4-treated mice. *P < 0.05 indicates a significant difference versus the Con group. c miR-200c levels in primary HSCs isolated from Con- and CCl4-treated mice. *P < 0.05 indicates a significant difference versus the Con group. d Representative images of H&E (upper), HA-binding protein (HABP) (middle), and F4/80 (lower) staining. e Quantification of F4/80+ staining. **P < 0.01 indicates a significant difference versus the WT-Con group; ##P < 0.01 indicates a significant difference versus the WT-CCl4 group. The data are presented as the mean ± SEM values. Significance was assessed by two-tailed Student’s t-test (a–c) and one-way ANOVA with Tukey’s post-hoc test (e).

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