Fig. 6: TNF-α-induced SASP mediates secretion of α-synuclein through lysosomal exocytosis. | Experimental & Molecular Medicine

Fig. 6: TNF-α-induced SASP mediates secretion of α-synuclein through lysosomal exocytosis.

From: TNF-α promotes α-synuclein propagation through stimulation of senescence-associated lysosomal exocytosis

Fig. 6

a–d Inhibition of lysosomal exocytosis reduced TNF-α-induced secretion of α-synuclein. a Representative western blot images of α-synuclein in Triton X-100-soluble (Tx-sol) and Triton X-100-soluble-insoluble (Tx-insol) fractions. The arrowhead indicates the quantified α-synuclein in Tx-sol. The line on the right side of the blot indicates the quantified area in Tx-insol. Quantification of α-synuclein in the Tx-sol (b) and Tx-insol (c) fractions. d Secreted α-synuclein, measured by ELISAs. e, f Effects of vacuolin-1 (e) and UC2288 (f) on TNF-α-induced secretion of β-hexosaminidase. All data are expressed as the mean ± SEM (*P < 0.05, **P < 0.005, ####P < 0.0001). Statistical significance was determined by two-way ANOVA with Tukey’s post hoc comparison between groups (b–f).

Back to article page