Table 1 Primer and oligonucleotide sequences used in this study.
Gene | Primer (sense) | Primer (antisense) |
|---|---|---|
hsa_circ_0047061 | CTCTTCTTCAAAAATGACCAGTGGG | TTCTTCTACACCATTTCGCCCT |
hsa_circ_0047060 | AAATGGAGCAGAAGTGCAGAACC | CAAACTTTCCTGCTCTTCATCCAA |
hsa_circ_0108012 | GGAGCAGAAGTGCAGATACT | GCAAACTTTCCTGCAAGCTTTTAT |
hsa_circ_0107991 | GCTGCAACTGGAACTCAACC | TTAACAGCCGCTTGCATGTC |
hsa_circ_0108010 | GAGCAGAAGTGCAGAACCTCA | CTGCATTGGAGCTAGTTCTGT |
hsa_circ_0108011 | AAATCACGACATGGCCCCTC | CGTGGTGGCTCACACTTCTT |
hsa_circ_0108016 | AGCTTGCTGTAGCACCAGTT | AGGGAATGTTTCTTCCTCTCCTT |
circROCK1-E3/E4 | TGGGTTGTTCAGATAAAGACACA | TGCACCTCTACCAATCACCT |
hsa_circ_0107997 | AATGAAGGACAGATGCGGGAG | CTGCTCCAGTTGCAGGGTTAG |
hsa_circ_0108001 | GTGGCAATGTGTGCTCGAAT | AGCATGTCTTGAGCCTCTTTTC |
hsa_circ_0047062 | GCCTGAAAAATGGGCACGAT | ATGCCTTACCTGTGAATAAAACC |
hsa_circ_0047056 | GTTTTTAAGCCACCCCCTGC | CACATCTCCTTGGGTTACAGGT |
hsa_circ_0107992 | CCTTAAAACACAGAGAAACTCTTGC | TGTTCTTCCAGAAGGCCTCG |
cANRIL | GCTGGGATTACAGGTGTGAGACACC | GAATCAGAATGAGGCTTATTCTTCTCATC |
ROCK1 mRNA | AAGAGGGCATTGTCACAGCA | AGCATCCAATCCATCCAGCA |
QKI mRNA | ACCTGCAGCAGAAGGAGAAG | AAGGCAAGGGCTGGTGATTT |
PTEN mRNA | TCCCAGACATGACAGCCATC | TGCTTTGAATCCAAAAACCTTACT |
GAPDH mRNA | AACGGATTTGGTCGTATTGGG | CCTGGAAGATGGTGATGGGAT |
β-Actin mRNA | GATTCCTATGTGGGCGACGA | TGTAGAAGGTGTGGTGCCAG |
ROCK1 intron 2 | TAGTGGGCGCAGTTAGACAC | CCCTGTGGAAGAACCTTGCT |
ROCK1 intron 4 | TGAAGCTGTGGCCATGTTACT | CAAAACCCCACTTGACCCCA |
miR-532-5p RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACGGTC | |
miR-532-5p | GCGGCGGCATGCCTTGAGTGTAG | ATCCAGTGCAGGGTCCGAGG |
U6 RT | CGAGCACAGAATCGCTTCACGAATTTGCGTGTCAT | |
U6 | CGAGCACAGAATCGCTTCA | CTCGCTTCGGCAGCACATAT |
siRNA | Sense | Antisense |
siNC | UUCUCCGAACGUGUCACGU dTdT | ACGUGACACGUUCGGAGAA dTdT |
sicircROCK1-1 | GTTGTTCAGATAAAGACACAAUU | TTGTGTCTTTATCTGAACAACUU |
sicircROCK1-2 | GGTTGTTCAGATAAAGACACAUU | TGTGTCTTTATCTGAACAACCUU |
siQKI-1 | GCAUCUAAAUGAAGAUUUAUU | TAAATCTTCATTTAGATGCUU |
siQKI-2 | GGUACUUUAUGUAUAAUUAUU | TAATTATACATAAAGTACCUU |
siPTEN-1 | GAUGAUACAUGUAAAUUAAUU | TTAATTTACATGTATCATCUU |
siPTEN-2 | GAAUGGAUUUGAUACUUUAUU | TAAAGTATCAAATCCATTCUU |
miR-532-5p mimic | CAUGCCUUGAGUGUAGGACCGU | GGUCCUACACUCAAGGCAUGUU |
miR-532-5p inhibitors | ACGGUCCUACACUCAAGGCAUG | |
miR NC | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
Inh NC | CAGUACUUUUGUGUAGUACAA | |
