Fig. 7: Chronic memantine treatment ameliorates vacuolation in the CA3 subfield of Xpnpep1–/– mice. | Experimental & Molecular Medicine

Fig. 7: Chronic memantine treatment ameliorates vacuolation in the CA3 subfield of Xpnpep1–/– mice.

From: Suppression of exaggerated NMDAR activity by memantine treatment ameliorates neurological and behavioral deficits in aminopeptidase P1-deficient mice

Fig. 7

a HE-stained images obtained at lower (top) and higher (bottom) magnifications show a significant reduction in the number of vacuoles in the CA3 region of Xpnpep1–/– mice following memantine treatment. Saline-treated Xpnpep1–/– mice exhibited dark neurons near confluent vacuoles in the CA3 stratum pyramidale. Scale bars, 500 μm (top) and 50 μm (bottom). b, c Quantification of the size (b) and number (c) of vacuoles in the hippocampal CA3 subfields. n = 4 mice in each group. b Genotype, F(1, 12) = 19.99, p < 0.001; treatment, F(1, 12) = 1.96, p = 0.18; interaction, F(1, 12) = 0.646, p = 0.43. c Genotype, F(1, 12) = 132.17, p < 0.001; treatment, F(1, 12) = 41.07, p < 0.001; interaction, F(1, 12) = 28.158, p < 0.001; two-way ANOVA with the Tukey multiple comparison test. d Histogram shows the size distribution of vacuoles in the hippocampal CA3 subfields. Data were pooled from four mice (12 slices) in each group.

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