Fig. 4: Integrated analysis of scRNA-seq and scATAC-seq in LR cells. | Experimental & Molecular Medicine

Fig. 4: Integrated analysis of scRNA-seq and scATAC-seq in LR cells.

From: DNA methylome and single-cell transcriptome analyses reveal CDA as a potential druggable target for ALK inhibitor–resistant lung cancer therapy

Fig. 4: Integrated analysis of scRNA-seq and scATAC-seq in LR cells.

a Left: Integrated scRNA-seq (n = 4566) and scATAC-seq (n = 6772) described by UMAP. Right: Annotated scATAC-seq clusters based on scRNA-seq. b Left: Calculation and classification of interaction scores. Right: Distribution of different genomic regions in the open chromatin region (OCR) groups. c Genomic region enrichment of annotations tool ontology analysis of OCRs. The P values shown are Bonferroni adjusted (n.d. not detected). d Transcription-factor motif enrichment analysis of OCRs. The most highly enriched motifs are shown. e ATAC signal for each cluster in the gene body of CDA. OCRs regulating CDA expression are displayed with the UCSC Genome Browser. f Relative CDA mRNA expression following knockdown of the indicated TFs using siRNAs. n = 3 independent experiments, mean ± SD, *P < 0.05, **P < 0.01 (Mann–Whitney U-test). g Schematic diagram of the regulatory roles of TEAD1, SMAD3, and FOXM1 in CDA activation in ceritinib-resistant cells.

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