Fig. 8: CLU and PRKD3 expressions in peripheral blood and a proposed working model. | Experimental & Molecular Medicine

Fig. 8: CLU and PRKD3 expressions in peripheral blood and a proposed working model.

From: Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling

Fig. 8

a, b ELISA analysis of CLU and PRKD3 protein levels in serum samples from LFH patients (n = 17) and non-LFH patients (n = 25). Data are expressed as the mean ± S.D. ns, not significant. c Mechanical stress increased CLU expression as well as TGF-β1, and TGF-β1 treatment also increased CLU expression as well as α-SMA and COL1A2. Further results revealed that CLU inhibited mechanical stress-stimulated and TGF-β1-induced SMAD3 activities through suppression of SMAD3 phosphorylation. The results also revealed that CLU inhibits phosphorylation and/or nuclear translocation by competitively binding to ALK5. In addition, they demonstrated that PRKD3 stabilizes CLU protein by inhibiting lysosomal distribution and degradation of CLU.

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