Fig. 6: Transplantation of NF-1 knockout neural stem cells (NSCs) facilitated axonal regeneration. | Experimental & Molecular Medicine

Fig. 6: Transplantation of NF-1 knockout neural stem cells (NSCs) facilitated axonal regeneration.

From: Transplanting neurofibromatosis-1 gene knockout neural stem cells improve functional recovery in rats with spinal cord injury by enhancing the mTORC2 pathway

Fig. 6: Transplantation of NF-1 knockout neural stem cells (NSCs) facilitated axonal regeneration.

a Electron microscopic (EM) visualization of visible demyelinated axons (red arrows) in different groups (scale bar = 2 µm). b Sections of toluidine blue staining showed myelinated and demyelinated axons in the different groups (scale bar = 20 µm). c Immunohistochemistry showed Fluorogold (FG)-labeled neurons in the T8 segment of the spinal cord, which indicates that neurogenesis can bridge the lesion epicenter (upper panels: lower magnification; lower panels: higher magnification) (scale bar = 100 µm). d Quantitative comparisons of proportions of myelinated axons based on DM and toluidine blue staining between the SCI+LV-vector NSC and SCI+LV-NF-1 NSC groups. e Quantitative comparisons of FG-labeled neurons at the T8 level between the SCI+LV-vector NSC and SCI+LV-NF-1 NSC groups. f Western blotting of CNPase, MBP, and GAPDH. g Quantitative comparisons of the expression of CNPase and MBP between the SCI+LV-vector NSC and SCI+LV-NF-1 NSC groups (data are expressed relative to GAPDH). *P < 0.05 compared with the LV-vector group.

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