Table 1 The reagents and materials used in cell experiments.

From: Transplanting neurofibromatosis-1 gene knockout neural stem cells improve functional recovery in rats with spinal cord injury by enhancing the mTORC2 pathway

Reagents and materials

Manufacturer

Dulbecco’s modified Eagle’s medium/F-12 nutrient mixture

Gibco, Grand Island, USA

B-27 supplement

Gibco, Grand Island, USA

Penicillin/streptomycin

Gibco, Grand Island, USA

L-glutamine

Gibco, Grand Island, USA

Fibroblast growth factor-2

Peprotech, Rocky Hill, USA

Epidermal growth factor

Peprotech, Rocky Hill, USA

Accutase

Thermo Fisher Scientific, Waltham, USA

Poly-L-lysine

Sigma, Germany

Neurobasal medium

Thermo Fisher Scientific, Waltham, USA

Tumor necrosis factor-alpha (TNF-α)

Peprotech, Rocky Hill, USA

T25 culture flask

Corning, Acton, USA

12-well plate

Corning, Acton, USA

6-well plate

Corning, Acton, USA

Coverslips

Thermo Fisher Scientific, Waltham, USA