Table 1 The reagents and materials used in cell experiments.
Reagents and materials | Manufacturer |
|---|---|
Dulbecco’s modified Eagle’s medium/F-12 nutrient mixture | Gibco, Grand Island, USA |
B-27 supplement | Gibco, Grand Island, USA |
Penicillin/streptomycin | Gibco, Grand Island, USA |
L-glutamine | Gibco, Grand Island, USA |
Fibroblast growth factor-2 | Peprotech, Rocky Hill, USA |
Epidermal growth factor | Peprotech, Rocky Hill, USA |
Accutase | Thermo Fisher Scientific, Waltham, USA |
Poly-L-lysine | Sigma, Germany |
Neurobasal medium | Thermo Fisher Scientific, Waltham, USA |
Tumor necrosis factor-alpha (TNF-α) | Peprotech, Rocky Hill, USA |
T25 culture flask | Corning, Acton, USA |
12-well plate | Corning, Acton, USA |
6-well plate | Corning, Acton, USA |
Coverslips | Thermo Fisher Scientific, Waltham, USA |