Fig. 2: Deletion of Cxxc5 ameliorates hepatic steatosis with metabolic improvement. | Experimental & Molecular Medicine

Fig. 2: Deletion of Cxxc5 ameliorates hepatic steatosis with metabolic improvement.

From: Metabolic improvement and liver regeneration by inhibiting CXXC5 function for non-alcoholic steatohepatitis treatment

Fig. 2

a–k Cxxc5+/+ and Cxxc5−/− mice were fed a HFD for 12 weeks and injected with CCl4 twice a week for the final 4 weeks to induce NASH (n = 7 per group). a Body weight. b GTT and ITT. c Fasting glucose. d Plasma insulin concentration in the overnight fasted state. e HOMA-IR. f Representative images of H&E staining of epiWAT and scWAT (upper panel). Quantification of CLSs and cell size in adipocytes (lower panel). Scale bars, 100 µm. g Representative histological analyses of images of liver tissue; H&E staining, ORO staining, and Pparγ staining. Scale bars, 100 µm. h Ratio of the ORO-positive area (left panel) and TG concentration (right panel) in the liver. i The scores of steatosis (left panel) and ballooning (right panel). j Serum concentrations of total cholesterol, HDL-cholesterol, TGs, leptin, and resistin. k Relative mRNA expression of genes involved in hepatic lipogenesis (n = 3). Data represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 determined by Student’s t test.

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