Fig. 3: Deletion of Cxxc5 ameliorates NASH progression. | Experimental & Molecular Medicine

Fig. 3: Deletion of Cxxc5 ameliorates NASH progression.

From: Metabolic improvement and liver regeneration by inhibiting CXXC5 function for non-alcoholic steatohepatitis treatment

Fig. 3

a–m Cxxc5+/+ and Cxxc5−/− mice were fed a HFD for 12 weeks and injected with CCl4 twice a week for the final 4 weeks to induce NASH (n = 7 per group). a Representative IHC images of F4/80- and Cd11b-stained liver tissues (left panel). Quantification of hCLS formation and F4/80- and Cd11b-positive areas (right panel). Scale bars, 100 µm. b Flow cytometry analyses of the expression of F4/80 and Cd11b (left panel). Percentage of F4/80+Cd11b+ cells (n = 3) (right panel). c Inflammation score. d Relative mRNA expression levels of genes involved in pro-inflammation (n = 3). e The NAFLD score. f Representative images of DHE-stained cells (left panel). Quantification of ROS formation by measuring DHE-positive cells in liver tissues (right panel). Scale bar, 100 µm. g TUNEL staining of the liver (top panel). Quantification of TUNEL-positive cells (bottom panel). Scale bar, 100 µm. h Relative mRNA expression of genes involved in cell death pathways (n = 3). i Representative IHC images for α-Sma, collagen I, desmin, and Sirius red staining of liver tissues. Scale bars, 100 µm. j Relative mRNA expression of genes involved in fibrogenesis (n = 3). k The fibrosis score. l, m Plasma concentrations of FFAs (l), AST and ALT (m). Data represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 determined by Student’s t test.

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