Fig. 7: KY19334 promotes regeneration of liver tissue in NASH-induced mice. | Experimental & Molecular Medicine

Fig. 7: KY19334 promotes regeneration of liver tissue in NASH-induced mice.

From: Metabolic improvement and liver regeneration by inhibiting CXXC5 function for non-alcoholic steatohepatitis treatment

Fig. 7

a–f C57BL/6 mice fed a NCD or HFD + CCl4 were orally administered KY19334, selonsertib, or ocaliva at 25 mg/kg/d for 4 weeks (n = 10 per group). The liver tissues were analyzed by various histochemical and biochemical methodologies. a Representative IHC images stained for Lgr5 or Cd133 (left panel). Quantification of Lgr5- and Cd133-positive cells (right panel). Scale bars, 100 µm. b Flow cytometry analyses of the expression of Lgr5 (n = 3). c Quantification of Lgr5-positive cells by flow cytometry analyses. d Relative mRNA expression of Lgr5 and liver progenitor cell markers (n = 3). e Representative IHC images stained for β-catenin or Lgr5. Scale bars, 100 µm. f Representative IHC images stained for Hnf4α or Ki67 (left panel). Quantification of Hnf4α+ and Ki67+ cells (right panel). Scale bars, 100 µm. Data represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 determined by Student’s t test.

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