Fig. 2: STAMBP interacts with RAI14. | Experimental & Molecular Medicine

Fig. 2: STAMBP interacts with RAI14.

From: The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability

Fig. 2: STAMBP interacts with RAI14.

a MDA-MB-231 cell lysates were incubated with beads conjugated with anti-STAMBP antibodies. Immunoprecipitates were analyzed by mass spectrometry. b Ambipolar ion peaks of the indicated proteins are shown. c Immunoblot analysis of the indicated protein expression in MDA-MB-231 and BT549 cells stably transfected with two individual STAMBP shRNAs or control shRNA. d Endogenous STAMBP and RAI14 interactions were detected by coimmunoprecipitation in MDA-MB-231 and BT549 cells. e The cellular location of STAMBP (green) and RAI14 (red) in MDA-MB-231 and BT549 cells was examined by immunofluorescence staining using STAMBP and RAI14 antibodies and visualized by fluorescence microscopy. DAPI (4′,6-diamidino-2-phenylindole) was used to stain nuclei. Scale bars: 10 μm.

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