Fig. 3: SCON is applicable and neutral in various vertebrate species.
From: SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
a–d Flow cytometry analysis of C6 (a), LLC-MK2 (b), PK-15 (c), and Vero (d) cells transfected with eGFP (red), eG-SCON-FP (blue), eG-SC-FP (yellow) or the empty vector or mCherry alone (gray). e Flow cytometry analysis comparing the GFP levels in various cell lines transfected with eG-DECAI-FP (gray) or eG-SCON-FP (yellow). *p < 0.001, from unpaired t test. f, g Xenopus and zebrafish embryos injected with eGFP, eG-SCON-FP, or eG-SC-FP constructs, which were imaged 24 h postinjection. A plasmid containing mCherry was used as an injection control. Scale bar, 500 μm.
