Fig. 2: EPB41L5 is the target gene of ZBTB7A in GBM cells.
From: ZBTB7A suppresses glioblastoma tumorigenesis through the transcriptional repression of EPB41L5
a Western blot analysis of ZBTB7A in shZBTB7A U87 cells. b Migration assay of shZBTB7A U87 cells after 12 h. c Transwell invasion assay of shZBTB7A U87 cells after 48 h. d Effect of ZBTB7A knockdown on colony formation in shZBTB7A U87 cells. e Cell proliferation assay was performed after shZBTB7A U87 and pCDH ZBTB7A U343 cells for 3 d. f Differentially expressed gene analysis of mRNA-seq of shZBTB7A U87 cells (red dots; upregulated genes, blue dots; downregulated genes, FC ± 1.5 and p value < 0.05). g Gene Ontology enrichment analysis for up- and downregulated genes scramble versus shZBTB7A. h, i GSEA revealed differences in the expression of genes involved in cell migration and EMT. j EMT-related core enrichment gene fold change value. NES (normalized enrichment score), FDR (fold discovery rate), **p < 0.01, ***p < 0.001.
