Fig. 3: ZBTB7A negatively regulates EPB41L5.
From: ZBTB7A suppresses glioblastoma tumorigenesis through the transcriptional repression of EPB41L5
a The Venn diagram shows an overlap between GSEA results. b Heatmap of the expression and enrichment of 18 overlapping genes in the GSEA results. c, d mRNA expression levels of 18 overlapping genes in shZBTB7A U87 cells and pCDH ZBTB7A U343 cells. e Negative correlation of the gene expression levels of MDK, IL33, and EPB41L5 with ZBTB7A (GlioVis). f, g mRNA expression levels of MDK, IL33, and EPB41L5 in shZBTB7A U87 cells and pCDH ZBTB7A U343 cells. h, i Western blot analysis of ZBTB7A and EPB41L5 in shZBTB7A U87 cells and pCDH ZBTB7A LN229 and U343 cells. j Western blot analysis of ZBTB7A and EPB41L5 in GBM cell lines. k EPB41L5 IHC staining in increasing grades of glioma using TMA blots. l IHC analysis of EPB41L5 expression in TMA blots. NB, n = 9; Grades I–II, n = 30; III–IV, n = 28. Scale bars: 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001.
