Fig. 2: USP15 expression and YAP1/TAZ signaling were significantly increased in lung tissues of SuHx-PH mice. | Experimental & Molecular Medicine

Fig. 2: USP15 expression and YAP1/TAZ signaling were significantly increased in lung tissues of SuHx-PH mice.

From: USP15 promotes pulmonary vascular remodeling in pulmonary hypertension in a YAP1/TAZ-dependent manner

Fig. 2

The SU5416/hypoxia (SuHx)-PH mouse model was used to mimic PH in vivo. a, b Real-time PCR and western blot analysis were performed to detect the pulmonary expression level of USP15 after modeling. c Representative immunofluorescence images of USP15 (red) and α-SMA (green) in lung tissues from the SuHx-PH mice and the sham mice. Scale bar: 50 μm. The white frame indicates the location of the pulmonary artery. d, e The protein levels of t-YAP1, t-TAZ, n-YAP1, and n-TAZ in the SuHx-PH and sham groups were determined by western blotting. f, g The mRNA levels of YAP1 and TAZ in lung tissue were detected by real-time PCR. h Coimmunostaining images of USP15 (red) and t-YAP1 (green) in lung sections from the sham and SuHx-PH groups. Scale bar: 100 μm. i Representative immunofluorescence results of USP15 (red) and t-TAZ (green) in lung sections from the sham and SuHx-PH groups. Scale bar: 100 μm. The white frame indicates the location of the pulmonary artery. Data are represented as the mean ± SD, n = 8 in each group.

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