Fig. 3: Progressive vasodilation associated with GBM invasion depends on VEGFR2 signaling.

a Schematic showing the cranial window chamber and experimental schedule with antibody treatment for intravital imaging. b–d Longitudinal vascular images in EGFRVIII GBM after control IgG or DC101 treatment. Vessels were stained for perfused lectin. c Magnified images of the boxed area in (b) at a shorter time interval. d Quantification of vessel diameter in IgG-treated (n = 4) and DC101-treated (n = 4) mice. Scale bars: 200 µm (b), 50 µm (c). ns not significant; *, P < 0.05; ***, P < 0.001.