Fig. 2: RNA-seq reveals an altered gene expression profile for inflammation in the absence of ERdj5.

a Venn diagram. The number of differentially expressed genes (DEGs) from colon samples extracted from 8-week-old female WT or ERdj5-KO mice (n = 3 per group) treated with or without 2% DSS. b KEGG pathway analysis of DEGs in colon samples extracted from 8-week-old female WT or ERdj5-KO mice (n = 3 per group) treated with or without 2% DSS. Pathways with statistical significance (P > 0.01) are shown. c GO analysis of major signaling pathways identified by RNA sequencing using the DAVID bioinformatics database. The top 10 pathways based on P values are shown, along with the number of genes with the respective ontology. d Heatmap of the DEGs associated with inflammation. The log₂ ratios of ERdj5 KO DSS/WT DSS are presented (blue, underexpression; red, overexpression). Values over 5 or under −5 were rounded. e Levels of IL-1β and IL-6 in colon homogenates (n = 6–8 per group). f Level of CXCL1 in colon homogenates (n = 6–8 per group). g Representative flow cytometric analysis of CD11b⁺Ly6G⁺ neutrophils and CD11b⁺Ly6C⁺ monocytes among pregated live CD45⁺IA-IE^-CD11c^- cells in the LP of the colon. The percentage of CD11b⁺Ly6G⁺ neutrophils is expressed as the mean ± SEM (n = 3–4 per group). *P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA followed by Tukey’s test.