Fig. 2: SAMHD1 accelerates the migration and invasion of human ccRCC cells. | Experimental & Molecular Medicine

Fig. 2: SAMHD1 accelerates the migration and invasion of human ccRCC cells.

From: SAMHD1-induced endosomal FAK signaling promotes human renal clear cell carcinoma metastasis by activating Rac1-mediated lamellipodia protrusion

Fig. 2

a Western blot analysis of SAMHD1 expression in normal renal epithelial cells (HK-2) and different RCC cell lines (Caki-1, Caki-2, SN12C, ACHN). Densitometric analysis of SAMHD1 protein expression levels relative to β-actin levels. b, c Wound-healing analysis of cell migration ability in SAMHD1-knockdown ACHN and SN12C cells or SAMHD1-overexpressed Caki-1 and Caki-2 cells over 24 h. Data are presented as the mean ± SD. The data shown are representative of three independent experiments. **p < 0.01; ***p < 0.001. d, e Transwell migration assay of cell migration capacity upon SAMHD1 knockdown in ACHN and SN12C cells or overexpression in Caki-1 and Caki-2 cells over 24 h. Data are presented as the mean ± SD. The data shown are representative of three independent experiments. ***p < 0.001. f, g Analysis of cell invasion ability upon SAMHD1 knockdown in ACHN and SN12C cells or overexpression in Caki-1 and Caki-2 cells. *p < 0.05; ***p < 0.001. Data are presented as the mean ± SD of three independent experiments.

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