Fig. 7: SAMHD1 contributes to endocytosis-dependent ccRCC cell migration. | Experimental & Molecular Medicine

Fig. 7: SAMHD1 contributes to endocytosis-dependent ccRCC cell migration.

From: SAMHD1-induced endosomal FAK signaling promotes human renal clear cell carcinoma metastasis by activating Rac1-mediated lamellipodia protrusion

Fig. 7

a IF staining with an anti-SAMHD1 antibody, an anti-EEA1 antibody, and DAPI in SAMHD1-overexpressed Caki-1 cells treated with 80 μM Dynasore over 24 h. b The number of EEA1 vesicles colocalized with SAMHD1 per cell was quantified and is shown on the graph (n = 30 cells). Data are presented as the mean ± SD. The data shown are representative of three independent experiments. ***p < 0.001. c IP assay was performed using Flag-tagged beads in Flag-SAMHD1-overexpressing Caki-1 cell lysates treated with 80 μM Dynasore over 24 h. d Phosphorylated cortactin (Y421) and total cortactin expression in SAMHD1-overexpressed Caki-1 cells treated with 80 μM Dynasore over 24 h and analyzed using western blotting. e, f Wound-healing assay of cell migration ability in SAMHD1-knockdown SN12C cells or in SAMHD1-overexpressed Caki-1 cells treated with 80 μM Dynasore. The relative wound-healing area was normalized to that of DMSO-treated controls. Data are presented as the mean ± SD. The data shown are representative of three independent experiments. ***p < 0.001. g G-LISA-based assay of GTP-bound Rac1 activity in SAMHD1-overexpressing Caki-1 cells treated with 10 μM NSC23766 or 80 μM Dynasore over 24 h. ***p < 0.001. Data are presented as the mean ± SD of three independent experiments.

Back to article page