Fig. 2: RNA-seq reveals Zeb1 as a target of MRTF-A.
From: An MRTF-A–ZEB1–IRF9 axis contributes to fibroblast–myofibroblast transition and renal fibrosis
A-F Primary murine renal fibroblasts were transduced with lentivirus carrying an MRTF-A expression vector (ΔN200) or empty vector (EV). RNA-seq was performed as described in the Methods. PCA plot (A). Volcano plot (B). GO analysis (C). KEGG analysis (D). GSEA (E). Heatmap of differentially expressed genes (F). G, H Primary murine renal fibroblasts were transduced with lentivirus carrying an MRTF-A expression vector (ΔN200) or empty vector (EV). Zeb1 expression was examined by qPCR and Western blotting. I, J Primary murine renal fibroblasts were isolated from MRTF-Af/f mice and transduced with adenovirus carrying Cre or GFP followed by treatment with TGF-β (2 ng/ml) for 24 h. Zeb1 expression was examined by qPCR and Western blot. K Primary murine renal fibroblasts were transduced with MRTF-A ΔN200 followed by transfection with the indicated siRNAs. Myofibroblast marker genes were examined by qPCR.
