Fig. 4: Zeb1 regulates FMyTin vitro and renal fibrosis in vivo.
From: An MRTF-A–ZEB1–IRF9 axis contributes to fibroblast–myofibroblast transition and renal fibrosis
A–D Primary murine renal fibroblasts were transfected with siRNA targeting Zeb1 or scrambled siRNA (SCR) followed by treatment with TGF-β (2 ng/ml) for 24 h. Myofibroblast marker gene expression was examined by qPCR (A). Cell proliferation and migration were examined by EdU incorporation (B) and Transwell assays (C), respectively. D Collagen contraction assay. E–I C57/B6 mice were injected via tail vein AAV2 carrying shRNA targeting Zeb1 (shZeb1) or control shRNA (shC) followed by the UUO procedure to induce renal fibrosis. The mice were sacrificed 2w after the surgery. (E) Scheme of the experimental protocol. F Plasma creatinine levels. G Plasma BUN levels. H Pro-fibrogenic gene expression levels were examined by qPCR. I Picrosirius red and Masson’s trichrome staining. N = 6-8 mice for each group.
