Fig. 7: Increased formation of cell proliferation-promoting metabolites derived from glutamine in SIM2KO cells.
From: Noncanonical role of singleminded-2s in mitochondrial respiratory chain formation in breast cancer
a, c Stable isotopic tracing of [13C5]-glutamine presented as the isotopic enrichment of a UMP, UDP, UTP, CMP, CDP, CTP, c proline (Pro), 2-hydroxyglutarate (2HG), and glutathione (GSH) in SIM2KO MCF7 cells. Data are presented as the means ± SEMs. **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed Student’s t test; n = 3 samples. Relative abundance of b all pyrimidine-containing metabolites (UMP, UDP, UTP, CMP, CDP, CTP), d Pro, 2HG, and GSH measured by addition of the area under all peaks of each metabolite and normalized to the total peak intensity of each sample. Data are expressed as SIM2KO MCF7 cell protein expression fold change compared to the protein expression level of the control cells ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t test; n = 3 samples. e Fluorescence signal from MitoSOX accumulation measured at 510 excitation and 580 emission. Data are plotted as the mean relative fluorescence ± SEM. *P < 0.05, two-tailed Student’s t test; n = 7 samples. f, g SIM2KO MCF7 cells were grown in 10 mM glucose-supplemented or b 1 mM glucose-supplemented medium with or without 2 mM glutamine. Cell counts were normalized to the number in glutamine-containing medium for each cell line and are represented as the number fold changes ± SEM. *P < 0.05, ****P < 0.0001, two-tailed Student’s t test; n = 3 samples.
