Fig. 6: Improvement of locomotor recovery by iNSC grafts after SCI in nude rats.
From: Human induced neural stem cells support functional recovery in spinal cord injury models
a Overview of the completely transected spinal cord of nude rats. b Representative GFP fluorescent immunolabeled cells in horizontal sections from the GFP-iNSC Group 10 weeks after SCI. c Quantification of the proportion of iNSC differentiation. d–i Images showing the expression of (d) MAP2, (e) NF200, (f) ChAT, (g) 5-HT, (h) GFAP, and (i) MBP at the transplant site of the GFP-iNSC Group 10 weeks after SCI. j Coexpression of GFP and SYP (arrowheads) in grafted cells. k, l Human axon terminals in contact with dendrites of host neurons (MAP2+ or ChAT+). m Propriospinal axons labeled with BDA regenerated into iNSC grafts. n Comparison of the mean BBB score in the vehicle (Con) and iNSC groups. Data are represented as the mean ± SD. *Denotes a statistically significant difference between the Con and iNSC groups, *p < 0.05, **p < 0.01 and ***p < 0.001. Scale bars, 200 μm (b, e–g, m); 10 μm (d, h–l).
