Fig. 6: USP8 binds to MUC12 and regulates its ubiquitination. | Experimental & Molecular Medicine

Fig. 6: USP8 binds to MUC12 and regulates its ubiquitination.

From: piRNA-1742 promotes renal cell carcinoma malignancy by regulating USP8 stability through binding to hnRNPU and thereby inhibiting MUC12 ubiquitination

Fig. 6

a, b The results of Co-IP and mass spectrometry confirmed that USP8 can directly bind to MUC12. c Expression of MUC12 in patients with different clinicopathological features by N stage and T stage. d The expression of MUC12, USP8, and hnRNPU in RCC samples by using western blotting. e Representative immunofluorescence images showing the colocalization of MUC12 and USP8; scale bar = 10 μm. f Western blotting was utilized to verify MUC12 expression following knockdown or overexpression of USP8. g A co-IP assay was used to verify the binding of USP8 to MUC12. h Western blotting was utilized to identify the expression of USP8 and MUC12 at different time points following CHX treatment. i Construction of a USP8-inactive mutant plasmid and determination of the MUC12 ubiquitination level via Co-IP after treatment with MG132. j Western blot analysis showed that the piR-1742-regulated expression of MUC12 could be rescued by USP8.

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