Fig. 5: Distinct metastatic patterns of ovarian cancer traced by mtDNA mutation.

Three representative cases (patient 3, patient 17, and patient 27) with distinct metastatic patterns (linear, mixed, and parallel) are shown. a Diagram of the spatial locations of samples in three patients. The abdominal cavity was divided into nine regions with blue dashed lines and represented by Roman numerals. The arrows indicate the locations and names of samples. b Heatmap showing the detailed pattern of somatic mutations and their respective heteroplasmy levels in different sampling locations of the three patients. The transverse axis is the sample name, with “p” and “m” representing the primary and metastatic sites, the longitudinal axis is the somatic mutation site, and the color in the square from white to red represents the heterogeneity level from low to high. c Phylogenetic tree of the three representative cases based on hierarchical clustering of mtDNA mutation and heteroplasmy. The length of the line segment represents the difference between branches, and the scale of the tree is on the left side. d Heatmap showing the level of pairwise correlation between the samples of the patients. The correlation values represented by colors within the squares are scaled on the right.