Fig. 4: MSC-EVs stimulate myofibroblast dedifferentiation through the transfer of miR-29c and miR-129 in vitro. | Experimental & Molecular Medicine

Fig. 4: MSC-EVs stimulate myofibroblast dedifferentiation through the transfer of miR-29c and miR-129 in vitro.

From: Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer

Fig. 4: MSC-EVs stimulate myofibroblast dedifferentiation through the transfer of miR-29c and miR-129 in vitro.

a Representative image of myofibroblast immunocytochemical analysis for αSMA expression. Scale bar = 200 μm. n = 4 biological independent experiments. b Bioinformatics analysis of specific and common targets for miR-29c and miR-129. c Western blot analysis of aSMA. n = 3 biological independent experiments. d Dot blot analysis of collagen type I. n = 3 biologically independent experiments. HEK293 cells were used as a negative control for antibody labeling. e Biotinylated microRNA pulldown assay for miR-29c and miR-129 and their target collagen type I. NTC no template control, n = 3 biological independent experiments.

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