Fig. 4: LPAR4 expression during mouse embryonic and postnatal development. | Experimental & Molecular Medicine

Fig. 4: LPAR4 expression during mouse embryonic and postnatal development.

From: SOX17-mediated LPAR4 expression plays a pivotal role in cardiac development and regeneration after myocardial infarction

Fig. 4Fig. 4

a Schematic representation of LPAR4 lineage tracing mouse embryo harvesting (embryonic Day 9.5 (E9.5), E10.5, E12.5, and E16.5). At least three mouse embryos were harvested for each time point. b Immunostaining for GFP and α-SA expression during LPAR4 lineage tracing mouse embryonic development. Merged images for GFP (green) and α-SA (red) from whole mouse embryos (E9.5, E10.5, E12.5, and E16.5). Green, GFP; red, αSA; blue, DAPI (nuclei). Scale bar, 500 μm. c Immunofluorescence analysis of embryonic heart, liver, and BM from LPAR4 lineage tracing mice at E10.5 and E12.5. Green, GFP; red, Sca1; white, SOX17; blue, DAPI (nuclei). Scale bar, 10 μm. All experiments were conducted at least in triplicate. d FACS analysis of heart, liver, and BM cells from LPAR4 lineage tracing mouse neonates (postnatal day 1). FACS analysis was performed using GFP (FITC) for LPAR4 expression and SOX17 (APC) or Sca1 (PE) staining. e Immunofluorescence analysis of LPAR4 lineage tracing for mouse neonatal heart, liver, and bone marrow cells. Green, GFP; red, Sca1; white, SOX17, blue, DAPI (nuclei).

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