Fig. 5: Ly6d inhibition suppresses lipid accumulation in hepatocytes.
From: LY6D is crucial for lipid accumulation and inflammation in nonalcoholic fatty liver disease
AML12 cells were transfected with siRNA, followed by incubation with 69.94 mM fructose for 24 h. si-Control control (NCC): scrambled siRNA-treated AML12 cells; si-Control+Fructose (NCF): scrambled siRNA-treated AML12 cells incubated with fructose; si-Ly6D: Ly6D knockdown AML12 cells; si-Ly6D+Fructose (LYF): Ly6D knockdown AML12 cells incubated with fructose. A Representative images of AML12 cells stained with Oil Red O. B, C qRT‒PCR analysis of hepatic gene expression related to lipogenesis B and lipolysis C (n = 6 in each group). D Heatmap visualizing the expression levels of genes related to NAFLD and NASH. E Bar chart summarizing the enriched KEGG pathways for DEGs between LYF and NCF. siRNA, short interfering RNA. The relative expression shown in B is presented as the mean ± SED, and the significance levels are *p < 0.05, **p < 0.01, and ***p < 0.001.
