Fig. 7: Single-cell RNA sequencing data from the livers of HFD-fed mice.
From: LY6D is crucial for lipid accumulation and inflammation in nonalcoholic fatty liver disease
The mice were intravenously injected with AAV-scrambled shRNA (Ctrl) or AAV-shLy6d (KD) and then fed a HFD. A UMAP plot for the integrated data. B Radar plot for the mean abundance of each cell type in the Ctrl and KD samples. C Violin plot for the log2-fold-change distribution of Ly6d, Lyz1, and Vsig4 in each cell type. D Volcano plot for B cells, mDCs, and pDCs. DEGs were defined as genes with abs (log2-FC)≥0.1 and p < 0.05. E Hierarchical clustering of enriched GO terms for downregulated DEGs in B cells and upregulated DEGs in T cells. F Network representation of similarities between enriched GO terms for upregulated DEGs in macrophages and heatmap for genes playing a key role in lipid and cholesterol metabolism. G Network representation of similarities between enriched GO terms for upregulated DEGs in Kupffer cells and heatmap for genes playing a key role in inflammatory responses. HFD high-fat diet, mDCs myeloid dendritic cells, pDCs plasmacytoid dendritic cells.
