Fig. 2: USP15 interacts with FABP1 and FABP4 and Perilipin1 and Perilipin2. | Experimental & Molecular Medicine

Fig. 2: USP15 interacts with FABP1 and FABP4 and Perilipin1 and Perilipin2.

From: Ablation of the deubiquitinase USP15 ameliorates nonalcoholic fatty liver disease and nonalcoholic steatohepatitis

Fig. 2

a USP15 interactome analysis. USP15 binding proteins were immunoprecipitated using a FLAG antibody and identified via mass spectrometry. b Interaction between endogenous USP15 and FABP1, FABP4, Perilipin1, and Perilipin2. The AML12 cells were immunoprecipitated using IgG and USP15 antibodies. c The interaction between exogenous USP15 and FABP4 or Perilipin1. HEK293 cells were transfected with FLAG-USP15, HA-FABP4, or HA-Perilipin1 plasmids and immunoprecipitated using a FLAG antibody. d GST pulldown assay showing a direct interaction between USP15 and FABP4 or Perilipin1. e Representative immunohistochemical staining for USP15 and Perilipin1 in patients with nonalcoholic fatty liver disease. The scale bar indicates 100 µm. f Overexpression of Perilipin1 was observed in NAFLD (p < 0.001) and NASH (p < 0.001). g There was a statistically significant correlation between USP15 and Perilipin1 in nonalcoholic fatty liver specimens (Pearson’s X2 test = 7.483, p = 0.006). ***p < 0.001 compared to normal.

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