Fig. 4: USP15 regulates the protein stability of FABP1/4 and Perilipin1/2 by deubiquitination. | Experimental & Molecular Medicine

Fig. 4: USP15 regulates the protein stability of FABP1/4 and Perilipin1/2 by deubiquitination.

From: Ablation of the deubiquitinase USP15 ameliorates nonalcoholic fatty liver disease and nonalcoholic steatohepatitis

Fig. 4

a Western blots of deubiquitination assays of FABP1/4 and Perilipin1/2. AML12 cells were transfected with or without siRNA against USP15 followed by 20 μM MG132 for 8 h. b Western blots of Ni2+ NTA pulldown show the deubiquitination of overexpressed FABP4 or Perilipin1 with 20 μM MG132 for 8 h. c Western blots of deubiquitination assays in HEK293 cells after transfection with either USP15 WT or C298A mutant followed by 20 μM MG132 for 8 h. d Effects of USP15 WT or C298A mutant on the protein stability of FABP4 or Perilipin1 in a USP15 dose-dependent manner. e Normalization of Western blots of protein levels in (d). Data were represented as the mean ± SEM. *p < 0.05, **p < 0.01 compared to USP15 WT.

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