Fig. 3: Identification of the behavior phenotype-specific module after CUS.

a Following CUS, each group was separated and mapped to different dimensions across the whole genome. b A volcano plot displaying differential gene expression between the SUS and RES groups. The x-axis represents the log₂ of the fold change in the gene [log₂ (fold change)], and the y-axis represents the −log₁₀ (p value). c Scatter plot displaying the differential gene expression between the SUS and RES groups. The genes highly expressed in SUS and RES animals are indicated by blue and red data points, respectively, while the genes that were not significantly differentially expressed in either of the groups are represented by gray data points. d CUS- or anhedonia susceptibility-related modules from 203 gene coexpression modules constructed via WGCNA. The modules were arranged clockwise from the modules with a large absolute value of the correlation coefficient with sample traits (CUS or anhedonic susceptibility). The module color is indicated by the circle’s outermost color, and the next color refers to the module group (orange: CUS-up, navy: CUS-down, blue: RES-up/SUS-down, red: RES-down/SUS-up). The expression patterns of eigengenes and summaries of the gene expression pattern of each module are colored and displayed in the order of SUS, RES, and CTRL from the inner line of the circle. e The intramodular coexpression network of a gene module specifically overexpressed in the SUS group was visualized. The thickness of the line indicates the strength of the correlation. f Gene Ontology enrichment analysis of the biological processes and cellular components of SUS-up module genes. g Syt4 expression in the M166 module after RNA sequencing (left, one-way ANOVA with Tukey’s test; F_2,6 = 18.30; p = 0.0028; n = 3) and qRT‒PCR validation after CUS (right, Kruskal‒Wallis test with Dunn’s test; H = 15.32; p = 0.0005; n = 10, 9, 9). ^**p < 0.01, ^***p < 0.001. The bar graphs show the mean ± SEM.