Fig. 4: Effects of Syt4 on mPFC activity and cell type/layer-specific localization. | Experimental & Molecular Medicine

Fig. 4: Effects of Syt4 on mPFC activity and cell type/layer-specific localization.

From: Synaptotagmin-4 induces anhedonic responses to chronic stress via BDNF signaling in the medial prefrontal cortex

Fig. 4

a Experimental procedures for capturing activated neurons during anhedonic behaviors. b Sucrose preference before (SPT1) and after i.p. injection of tamoxifen (SPT2; two-way ANOVA with Fisher’s LSD test: group effect, F2,18 = 12.82, p = 0.0003; time effect, F1,18 = 0.3658, p = 0.5528; interaction, F2,18 = 0.7190, p = 0.5007, n = 4). c Representative images of Fos+ cells induced by Syt4 overexpression (Syt4-OE) and SCUS. d The percentage of Fos+ cells relative to DAPI-stained cells in each group following SCUS (one-way ANOVA with Tukey’s test, F2,6 = 32.57, p = 0.0006, n = 3, 3, 3). e The percentage of Fos+ cells relative to EGFP+ cells in each group following SCUS (one-way ANOVA with Tukey’s test, F2,6 = 10,94 p = 0.0100, n = 3, 3, 3). f Cell-type enrichment analysis of the M166 module, which includes Syt4 as a hub gene (Fisher’s exact test). g A representative image of fluorescence in situ hybridization for Syt4 localization. h Representative images depicting the expression of Syt4, vGluT1, and vGAT in the PFC were captured for each group. i Cell-type specificity of Syt4 expression in the PFC (unpaired t test, t6 = 5.548, p = 0.0014, n = 4). j Syt4 expression in the PFC according to CUS phenotype (one-way ANOVA with Tukey’s test, F2,9 = 23.83, p = 0.0003, n = 4). *p < 0.05, **p < 0.01, ***p < 0.001. The bar graphs show the mean ± SEM. Glu glutamatergic neuron, GABA GABAergic neuron, Oligo oligodendrocyte, Astro astrocyte, Endo endothelial cell, SMC/Peri smooth muscle cell/pericyte, VLMC vascular and leptomeningeal cell, Micro/PVM microglia/perivascular macrophage, L2/3 layer 2/3 glutamatergic neuron, L4/5/6 Car3 layer 4/5/6 or Car3+ glutamatergic neuron, PT pyramidal tract glutamatergic neuron, NP/CT/L6b near-projecting/corticothalamic/layer 6b glutamatergic neuron, CR Cajal-Retzius glutamatergic neuron, CGE caudal ganglionic eminence-originated GABAergic neuron, MGE medial ganglionic eminence-originated GABAergic neuron, Meis2 Meis2+ GABAergic neuron.

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