Fig. 5: SAA1 increases noncanonical inflammasome-mediated lethality and pyroptosis. | Experimental & Molecular Medicine

Fig. 5: SAA1 increases noncanonical inflammasome-mediated lethality and pyroptosis.

From: Deficiency of circadian clock gene Bmal1 exacerbates noncanonical inflammasome-mediated pyroptosis and lethality via Rev-erbα-C/EBPβ-SAA1 axis

Fig. 5

a Survival of mice intraperitoneally injected with poly(I:C) (10 mg/kg) and challenged with LPS (1 mg/kg) in the presence or absence of SAA1 (50 μg/kg). (n = 14; female). b Quantification of LDH in culture supernatants of BMDMs treated with SAA1 (1 μg/ml, 3 h) alone or followed by E. coli infection (MOI 20, 2 h) (n = 3). c Quantification of PI uptake by BMDMs treated with SAA1 (1 μg/ml, 3 h), followed by E. coli infection (MOI 20, 1 h), in the presence of PI (n = 3). d Representative immunoblot of BMDMs treated with SAA1 (1 μg/ml, 3 h), followed by E. coli infection (MOI 20, 2 h) (n = 3). e Representative immunoblot of BMDMs treated with SAA1 (1 μg/ml, 3 h) or LPS (100 ng/ml, 3 h), followed by ATP treatment (3 mM, 1 h) (n = 3). f Representative immunofluorescence images of BMDMs treated with SAA1 (1 μg/ml) and LLME (0.4 mM) for 3 h. (n = 3) Bar = 20 μM. Data represent the mean ± SEM. *P < 0.05, **P < 0.01.

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