Fig. 1: The proportion of M1 MΦs is increased in degenerated NP tissue. | Experimental & Molecular Medicine

Fig. 1: The proportion of M1 MΦs is increased in degenerated NP tissue.

From: Nucleus pulposus cells regulate macrophages in degenerated intervertebral discs via the integrated stress response-mediated CCL2/7-CCR2 signaling pathway

Fig. 1

a Heatmap showing immune cell abundance in bulk RNA-seq datasets (GSE167199; GSE176205). b Quantitative analysis of related MΦs using the xCell score. c T-SNE analysis plot of single-cell transcriptome datasets (GSE165722; GSE199866) identified by SingleR. MΦ subpopulations are annotated by Sctype based on annotated marker genes. d Quantitative analysis of MΦ percentages in scRNA datasets. e MRI images of IDD patient samples. The red arrow indicates the surgical site. f Immunohistochemical staining of MΦ markers (CD68, CD86, CD206) in control and degenerated human NP tissue. Experiments were performed at least 3 times, and the data are presented as the means ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant, with Student’s t test.

Back to article page