Fig. 1: Single-cell characterization of the disrupted transcriptional profiles in type I NBs induced by Baf KD.

a A schematic diagram of the combinatorial indexing-based scRNA-seq pipeline for third-instar larval brains. b UMAP plots showing all cells individually colored according to their cell type (top) or treatment condition (bottom). c Heatmap displaying the Z scores of the mean normalized expression of cell type-specific markers in each cell type. d Bar plot showing the AUC of each cell type indicating the separability of the control and Baf KD cells according to their transcriptomes. The number of each cell type and DEGs are shown in the additional table (right). e GO enrichment analysis of upregulated (orange) and downregulated (green) genes in type I NBs lacking Baf. f Heatmap showing the Z scores of the mean normalized expression of neurite-related genes that were upregulated in type I NBs lacking Baf. g Representative images of cultured primary type I NBs that were isolated from control larval brains (top, ase-Gal4/+;UAS-mCD8GFP,UAS-H2A.mRFP/+) or from larval brains expressing Baf RNAi (bottom, ase-Gal4/+;UAS-mCD8GFP,UAS-H2A.mRFP/UAS-Baf RNAi). Cultured primary type I NBs were visualized by the expression of the fluorescent plasma membrane marker protein, mCD8GFP. The left three panels show serial z-stack images, and the right panels show images processed with maximum intensity projection (M.I.P.) (Scale bar, 5 μm).