Fig. 2: Aberrant anchoring of heterochromatin to the INM and perturbed formation of type I NBs and their neural progenies induced by Baf deficiency. | Experimental & Molecular Medicine

Fig. 2: Aberrant anchoring of heterochromatin to the INM and perturbed formation of type I NBs and their neural progenies induced by Baf deficiency.

From: Baf-mediated transcriptional regulation of teashirt is essential for the development of neural progenitor cell lineages

Fig. 2

a Representative images of immunostaining for H3K9me3 with Hoechst staining in cultured primary control type I NBs and type I NBs expressing Baf RNAi that were isolated from the brains of third-instar larvae (ase-Gal4/+;UAS-mCD8GFP/+ and ase-Gal4/+;UAS-mCD8GFP/UAS-Baf RNAi). NBs are visualized by the expression of the fluorescent plasma membrane marker protein mCD8GFP. The white dashed lines in the panels indicate the outlines of the inner nuclear membrane (Scale bar, 10 μm). b Representative images of control type I NBs (top, ase-Gal4/+;UAS-mCD8GFP/+) or type I NBs expressing Baf RNAi (bottom, ase-Gal4/+;UAS-mCD8GFP/UAS-Baf RNAi) in the brains of third-instar larvae. NBs are visualized by the expression of the fluorescent plasma membrane marker protein mCD8GFP. The white dashed lines in the left panels indicate the outlines of the brain lobes. Magnified images of type I NBs indicated by red arrowheads in the left panel are presented in the right panels (Scale bars, 20 μm). c Quantification of the number of type I NBs located in the apical cortex of third-instar larval brain lobes expressing the transgenes indicated in (b). **P < 1.0 × 10−2 according to Student’s t-test; error bars, mean ± SEM; the number of brain lobes tested was as follows: Control = 3 brain lobes, Baf KD = 3 brain lobes. d Quantification of the number of neural progenies derived from type I NBs located in the apical cortex of third-instar larval brain lobes expressing the transgenes indicated in (b). ****P < 1.0 × 10−4 according to Student’s t-test; error bars, mean ± SEM; the number of brain lobes tested was as follows: Control = 10 NBs, Baf KD = 6 NBs. e Representative images of BrdU (magenta) staining in control type I NBs and type I NBs expressing Baf RNAi in the brains of third-instar larvae. (ase-Gal4/+;UAS-mCD8GFP/+ and ase-Gal4/+;UAS-mCD8GFP/UAS-Baf RNAi). Magnified images of the red squares in the leftmost panels are presented in the second left panels. The white dashed lines in the panels indicate the outlines of the NBs (Scale bars, 20 μm). f Quantification of the proportion of BrdU-positive type I NBs to total type I NBs located in the apical cortex of third-instar larval brain lobes expressing the transgenes indicated in (e). ****P < 1.0 × 10−4 according to Student’s t-test; error bars, mean ± SEM; the number of brains tested is as follows: Control = 10 brain lobes, Baf KD = 7 brain lobes.

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