Fig. 6: Isoniazid (INH) and copper (Cu) induce mitochondrial dysfunction in HepG2 cells.
From: Synergistic toxicity with copper contributes to NAT2-associated isoniazid toxicity
Measurements of mitochondrial ROS generation using FACS analyses with MitoSOX. Examples of FACS dot plots are shown (a), and results of multiple experiments are presented in (b, n = 3). The percentage of MitoSOX-positive cells was greatly increased in ATP7B KO cells treated with INH (1000 µM) or Cu (500 µM) for 24 h. Measurement of functional mitochondria (%) using the MitoTracker system. MitoTracker CMXRos stains mitochondria in live cells, and its relative accumulation compared to MitoTracker FM depends on membrane potential. Examples of FACS dot plots are shown (c), and results of multiple experiments are presented in (d, n = 3). The percentage of dysfunctional mitochondria was greatly increased in ATP7B KO cells treated with INH or Cu for 24 h. Bar graph data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by multiple t tests with FDR correction. FCCP (25 μM) was used as a positive control.
