Fig. 2: IL-10^GFP+ ILCs ameliorate OXZ-induced CHS.

a Representative flow cytometry images of IL-10⁺ and/or GFP⁺ ILCs from the spleens of WT or tiger mice. Lin⁻CD45⁺CD127⁺ ILCs were gated for IL-10 versus GFP expression. b GFP⁺ ILCs were isolated from tiger mice and i.v. transferred into WT mice (−1 day before sensitization). Four days after OXZ challenge, immunofluorescence staining was performed to detect infiltrated GFP⁺ ILCs in the spleen, cLN, and ear tissues by using an antibody against GFP (green). Scale bar, 100 μm. The results are presented as representative images from three independent experiments (a, b) (n = 2 per group for each experiment). c, d Ear thickness (c) and representative H&E image (d) of the ears of WT CHS mice after the transfer of GFP⁺ or GFP⁻ ILCs (n = 6). The data are presented as representative images (c) and the mean ± SEM (d) from two independent experiments (n = 3 per group for each experiment). *p < 0.05 and **p < 0.01 versus the WT OXZ + PBS-treated group according to Student’s t test. e, f Histograms showing the numbers of infiltrating lymphocytes, monocytes, granulocytes (e), CD4⁺ T cells, and CD8⁺ T cells (f) in the ear (n = 4–6) are shown. The data are expressed as the mean ± SEM from two independent experiments (n ≥ 2 per group for each experiment). *p < 0.05, **p < 0.01, and n.s., not significant versus the WT OXZ + PBS-treated group according to Student’s t test.